2qyf

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Template:STRUCTURE 2qyf

Contents 1 Crystal structure of the Mad2/p31(comet)/Mad2-binding peptide ternary complex 2 Function 3 About this Structure 4 Reference

Crystal structure of the Mad2/p31(comet)/Mad2-binding peptide ternary complex

Template:ABSTRACT PUBMED 18022368

Function

[MD2L1_HUMAN] Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.^{[1] [2] [3]} [MD2BP_HUMAN] May function to silence the spindle checkpoint and allow mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex.^{[4] [5]}

About this Structure

2qyf is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

• Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X. p31comet blocks Mad2 activation through structural mimicry. Cell. 2007 Nov 16;131(4):744-55. PMID:18022368 doi:10.1016/j.cell.2007.08.048

1. ↑ Luo X, Fang G, Coldiron M, Lin Y, Yu H, Kirschner MW, Wagner G. Structure of the Mad2 spindle assembly checkpoint protein and its interaction with Cdc20. Nat Struct Biol. 2000 Mar;7(3):224-9. PMID:10700282 doi:10.1038/73338
2. ↑ Luo X, Tang Z, Rizo J, Yu H. The Mad2 spindle checkpoint protein undergoes similar major conformational changes upon binding to either Mad1 or Cdc20. Mol Cell. 2002 Jan;9(1):59-71. PMID:11804586
3. ↑ Luo X, Tang Z, Xia G, Wassmann K, Matsumoto T, Rizo J, Yu H. The Mad2 spindle checkpoint protein has two distinct natively folded states. Nat Struct Mol Biol. 2004 Apr;11(4):338-45. Epub 2004 Mar 14. PMID:15024386 doi:10.1038/nsmb748
4. ↑ Habu T, Kim SH, Weinstein J, Matsumoto T. Identification of a MAD2-binding protein, CMT2, and its role in mitosis. EMBO J. 2002 Dec 2;21(23):6419-28. PMID:12456649
5. ↑ Yang M, Li B, Tomchick DR, Machius M, Rizo J, Yu H, Luo X. p31comet blocks Mad2 activation through structural mimicry. Cell. 2007 Nov 16;131(4):744-55. PMID:18022368 doi:10.1016/j.cell.2007.08.048