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1w8f
From Proteopedia
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PSEUDOMONAS AERUGINOSA LECTIN II (PA-IIL) COMPLEXED WITH LACTO-N-NEO-FUCOPENTAOSE V(LNPFV)
Overview
One of the mechanisms contributing to the protection by breast-feeding of, the newborn against enteric diseases is related to the ability of human, milk oligosaccharides to prevent the attachment of pathogenic bacteria to, the duodenual epithelium. Indeed, a variety of fucosylated, oligosaccharides, specific to human milk, form part of the innate immune, system. In the present study, we demonstrate the specific blocking of, PA-IIL, a fucose-binding lectin of the human pathogen Pseudomonas, aeruginosa, by milk oligosaccharides. Two fucosylated epitopes, Lewis a, and 3-fucosyl-lactose (Lewis x glucose analogue) bind to the lectin with, dissociation constants of 2.2x10(-7) M and 3.6x10(-7) M respectively., Thermodynamic studies indicate that these interactions are dominated by, enthalpy. The ... [(full description)]
About this Structure
1W8F is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with SO4, CA and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural basis for the interaction between human milk oligosaccharides and the bacterial lectin PA-IIL of Pseudomonas aeruginosa., Perret S, Sabin C, Dumon C, Pokorna M, Gautier C, Galanina O, Ilia S, Bovin N, Nicaise M, Desmadril M, Gilboa-Garber N, Wimmerova M, Mitchell EP, Imberty A, Biochem J. 2005 Jul 15;389(Pt 2):325-32. PMID:15790314
Page seeded by OCA on Tue Oct 30 16:28:17 2007
Categories: Pseudomonas aeruginosa | Single protein | Bovin, N. | Budova, M. | Desmadril, M. | Dumon, C. | Galanina, O. | Gautier, C. | Gilboa-Garber, N. | Ilia, S. | Imberty, A. | Mitchell, E.P. | Nicaise, M. | Perret, S. | Sabin, C. | Wimmerova, M. | CA | GOL | SO4 | Cystic firosis | Infection of lungs | Lectin | Pentassacharide
