1ytw
From Proteopedia
|
YERSINIA PTPASE COMPLEXED WITH TUNGSTATE
Overview
X-ray crystal structures of the Yersinia tyrosine phosphatase (PTPase) in, complex with tungstate and nitrate have been solved to 2. 4-A resolution., Tetrahedral tungstate, WO42-, is a competitive inhibitor of the enzyme and, is isosteric with the substrate and product of the catalyzed reaction., Planar nitrate, NO3-, is isosteric with the PO3 moiety of a, phosphotransfer transition state. The crystal structures of the Yersinia, PTPase with and without ligands, together with biochemical data, permit, modeling of key steps along the reaction pathway. These energy-minimized, models are consistent with a general acid-catalyzed, in-line displacement, of the phosphate moiety to Cys403 on the enzyme, followed by attack by a, nucleophilic water molecule to release orthophosphate. This ... [(full description)]
About this Structure
1YTW is a [Single protein] structure of sequence from [Yersinia enterocolitica] with WO4 and SO4 as [ligands]. Active as [Protein-tyrosine-phosphatase], with EC number [3.1.3.48]. Structure known Active Sites: PL and WPD. Full crystallographic information is available from [OCA].
Reference
The X-ray crystal structures of Yersinia tyrosine phosphatase with bound tungstate and nitrate. Mechanistic implications., Fauman EB, Yuvaniyama C, Schubert HL, Stuckey JA, Saper MA, J Biol Chem. 1996 Aug 2;271(31):18780-8. PMID:8702535
Page seeded by OCA on Tue Oct 30 16:33:35 2007
