RuBisCO
From Proteopedia
Ribulose-1,5-bisphosphate carboxylase oxygenase – RuBisCO (RBCO) catalyzes the first step in carbon fixation. RBCO carboxylates or oxygenates ribulose-1,5-bisphosphate (RUBP) with CO2 or O2, respectively. RBCO from flowering plants usually consists of eight large subunits and eight small subunits. 2-carboxylarabinitol-1,5-bisphosphate (2-CABP) is an inhibitor of RCBO. Some additional details in Ribulose-1,5-bisphosphate carboxylase/oxygenase.
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Quaternery Structure
The Rubisco is shown in spacefill with its 8 large subunits in shades of blue and and its 8 small subunits in shades of yellow. The large subunits are arranged in head-to-toe pairs like staves in a barrel and the small subunits are arranged at the ends of the barrel. The large subunits contain the active sites and the function of the small subunits is not understood.
Active Site Structure
Each , has located in their interface. The subunits are shown in cartoon and the active sites are occupied by RUBP in CPK spacefill).
The active site of each subunit is formed by an α-β barrel located in the N-terminal lobe. Here the , and here is the α-β barrel of that subunit.
3D Structures of RuBisCO
Updated February 2013
RuBisCO
3rg6, 1rbl – SeRBCO – Synechococcus elongatus
2ybv - RBCO – Thermosynechococcus elongatus
3qfw - RBCO large subunit – Rhodopseudomonas palustris
1uzh, 1gk8 – CrRBCO – Chlamydomonas reinhardtii
1uw9, 1uwa – CrRBCO (mutant)
1svd – RBCD – Halothiobacillus neapolitanus
1bxn – RBCO – Cupriavidus necator
1aus - spRBCO – spinach
1rba - RrRBCO (mutant) – Rhododpirillum rubrum
5rub - RrRBCO
2wvw – RBCO – Anabena – Cryo EM
2vdh, 2vdi, 2v67, 2v68, 2v63, 2v69, 2v6a - CrRBCO (mutant)
1mlv - pRBCO LSMT – pea
2cxe, 2cwx – PhRBCO - Pyrococcus horikoshii
1uzd – CrRBCO/spRBCO
1geh – TkRBCO – Thermococcus kodakaraensis
1iwa - GpRBCO – Galdieria partita
1tel – RBCO large subunit – Chlorobium tepidum
1rld, 3rub, 3t15, 3zw6, 4rub – tRBCO – tobacco
3thg – RBCO – creosote bush
4hhh – RBCO - pea
RuBisCO complex with inhibitor 2-CABP
3kdn, 3a12 – TkRBCO III + 2-CABP
3kdo, 3a13 - TkRBCO III (mutant) + 2-CABP
1ir2 - CrRBCO + 2-CABP
1upm, 1upp, 1rbo, 3ruc, 8ruc - spRBCO + 2-CABP + cation
1ir1 - spRBCO + 2-CABP + CO2 + Mg
1wdd – rRBCO + 2-CABP – rice
1bwv - GpRBCO + 2-CABP
1rlc - tRBCO + 2-CABP
RuBisCO complex with product
1aa1 – spRBCO + phosphoglycerate
1rus - RrRBCO + phosphoglycerate
RuBisCO complex with substrate
1rcx, 1rxo – spRBCO + ribulose-1,5-bisphosphate
9rub - RrRBCO + ribulose-1,5-bisphosphate
1rsc - SeRBCO + xylulose-1,5-bisphosphate
1rco - spRBCO + xylulose-diol-1,5-bisphosphate
3zxw - SeRBCO + carboxyarabinitol-1,5-bisphosphate
RuBisCO complexes
2h21 – pRBCO LSMT + AdoMet
2h23 - pRBCO LSMT + AdoHcy
2h2e, 1ozv, 1p0y - pRBCO LSMT + AdoMet + lysine
2h2j - pRBCO LSMT + sinefungin
2d69 – PhRBCO + sulfate
2rus - RrRBCO + CO2 + Mg
4f0h – GsRBCO + O2 – Galdieria sulphuraria
4f0k - GsRBCO + CO2 + Mg
4f0m - GsRBCO + Mg
1ej7 – tRBCO + phosphate
3axk – rRBCO + NADP
3axm – rRBCO + 6PG
Proteopedia Page Contributors and Editors (what is this?)
Michal Harel, Alice Harmon, Joel L. Sussman, Alexander Berchansky
