3a3v

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Template:STRUCTURE 3a3v

Contents

Crystal structure of reducing-end-xylose releasing exo-oligoxylanase Y198F mutant

Template:ABSTRACT PUBMED 19819900

Function

[REOX_BACHD] Hydrolyzes xylooligosaccharides with a degree of polymerization of greater than or equal to 3, releasing xylose from the reducing end. Only hydrolyzes the beta anomers of xylooligosaccharides, with inversion of anomeric configuration. Hydrolyzes the glucose and xylose-based trisaccharides where xylose is located at the -1 subsite, GXX, XXG and GXG. Does not hydrolyze xylan, chitosan, lichenan, curdlan or carboxymethylcellulose.[1]

About this Structure

3a3v is a 1 chain structure with sequence from "bacillus_alcalophilus_subsp._halodurans"_boyer_et_al._1973 "bacillus alcalophilus subsp. halodurans" boyer et al. 1973. Full crystallographic information is available from OCA.

Reference

  • Hidaka M, Fushinobu S, Honda Y, Wakagi T, Shoun H, Kitaoka M. Structural explanation for the acquisition of glycosynthase activity. J Biochem. 2010 Feb;147(2):237-44. Epub 2009 Oct 9. PMID:19819900 doi:10.1093/jb/mvp159
  1. Honda Y, Kitaoka M. A family 8 glycoside hydrolase from Bacillus halodurans C-125 (BH2105) is a reducing end xylose-releasing exo-oligoxylanase. J Biol Chem. 2004 Dec 31;279(53):55097-103. Epub 2004 Oct 18. PMID:15491996 doi:10.1074/jbc.M409832200

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