2z6o
From Proteopedia
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Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1
Overview
Ubiquitin and ubiquitin-like protein-conjugating enzymes play central roles in posttranslational modification processes. The ubiquitin-fold modifier 1 (Ufm1), one of a variety of ubiquitin-like modifiers, is covalently attached to target proteins via Uba5 and Ufm1-conjugating enzyme 1 (Ufc1), which are analogous to the E1 and E2 ubiquitylation enzymes. As Ufm1-related proteins are conserved in metazoa and plants, the Ufm1 system likely plays important roles in various multicellular organisms. Herein, we report the X-ray structure of human Ufc1 determined at 1.6 A resolution. The Ufc1 structure comprises a canonical E2 domain and an additional N-terminal domain. The Uba5 binding site on Ufc1 was assigned by structural comparison of Ufc1 and Ubc12 and related mutational analyses. In addition, we show that the N-terminal unique domain of Ufc1 contributes to thermal stability.
About this Structure
2Z6O is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Ubiquitin--protein ligase, with EC number 6.3.2.19 Full crystallographic information is available from OCA.
Reference
Crystal structure of Ufc1, the Ufm1-conjugating enzyme., Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T, Biochem Biophys Res Commun. 2007 Nov 3;362(4):1079-84. Epub 2007 Aug 30. PMID:17825256
Page seeded by OCA on Thu Feb 21 19:00:32 2008
Categories: Homo sapiens | Single protein | Ubiquitin--protein ligase | Kawakami, T. | Komatsu, M. | Kominami, E. | Mizushima ,T. | Ogasahara, K. | Ozaki, Y. | Suzuki, A. | Tanaka, K. | Tatsumi, K. | Yamane, T. | MG | Ligase | Polymorphism | Ubiquitin | Ubl | Ubl conjugation pathway | Ufc1 | Ufm1
