User:Alexander Rudecki/Sandbox 1

From Proteopedia

Introduction

Drosophila melanogaster glutaminyl cyclase (DromeQC but also known as CG10487 CG32412 or Dmel\CG32412) is a globular protein part of the α/β-hydrolase superfamily. DromeQC is an aminoacyltransferase (EC 2.3.2.5) that acts on N-terminal glutamine or glutamate residues, producing a stable cap resistant to protease degradation. The human orthologue of DromeQC (hQC) has been implicated in stabilizing amyloid Aβ peptides involved in neurodegenerative disorders such as Alzheimers^[1]. It has been shown that DromeQC has a similar overall fold to hQC, as well as a conserved active site^[2]. Thus DromeQC is an attractive candidate for transgenic models and mechanistic studies.

Gene

DromeQC is encoded by chromosome 3, locus 64F4-64F5 in the "D. melanogaster" genome. It is transcribed into a 1622 nucleotide transcript containing 6 exons. The translated protein contains 340 residues corresponding to a M=38,028 Da. It contains a signal sequence which suggests it is involved in the secretory pathway ^[2].

Structure

References

1. ↑ Schilling S, Zeitschel U, Hoffmann T, Heiser U, Francke M, Kehlen A, Holzer M, Hutter-Paier B, Prokesch M, Windisch M, Jagla W, Schlenzig D, Lindner C, Rudolph T, Reuter G, Cynis H, Montag D, Demuth HU, Rossner S. Glutaminyl cyclase inhibition attenuates pyroglutamate Abeta and Alzheimer's disease-like pathology. Nat Med. 2008 Oct;14(10):1106-11. doi: 10.1038/nm.1872. Epub 2008 Sep 28. PMID:18836460 doi:http://dx.doi.org/10.1038/nm.1872
2. ↑ ^{2.0 2.1} Koch B, Kolenko P, Buchholz M, Ruiz Carrillo D, Parthier C, Wermann M, Rahfeld JU, Reuter G, Schilling S, Stubbs MT, Demuth HU. Crystal Structures of Glutaminyl Cyclases from Drosophila melanogaster Reveal Active Site Conservation between Insect and Mammalian QCs. Biochemistry. 2012 Aug 16. PMID:22897232 doi:10.1021/bi300687g