NAD(P) transhydrogenase (PTH) catalyzes the conversion of NADP and NADH to NADPH and NAD. The reaction is coupled with proton translocation across the cell membrane while the enzyme undergoes conformational change. PTH uses FAD as cofactor. PTH is composed of 3 domains.:
- Domain I binds NAD(+)/NADH.
- Domain II is a membrane-spanning domain.
- Domain III binds NADP(+)/NADPH.
PTH is composed of 2 subunits. Subunit beta contains domain III and part of II.
3D structures of NAD(P) transhydrogenase
Updated on 30-March-2014
PTH domain I
1l7d – RrPTH domain I – Rhodospirillum rubrum
1l7e – RrPTH domain I + NAD
1x13 – EcPTH domain I – Escherichia coli
1x14, 1x15 – EcPTH domain I + NAD
4dio – PTH domain I – Sinorhizobium meliloti
PTH domain III
1e3t – RrPTH domain III + NADP - NMR
1pno – RrPTH domain III + NADP
1pnq – RrPTH domain III + NADPH
1d4o – bPTH domain III + NADP - bovine
1djl, 1u31 – hPTH domain III + NADP - human
1pt9 – hPTH domain III + NADP analog
PTH domain I+III
1hzz, 1u28, 1u2d, 1xlt – RrPTH domain I+III + NADP + NAD
1f8g – RrPTH domain I+III + NAD
1nm5, 2fr8, 2fsv – RrPTH domain I+III (mutant) + NADP + NAD
1ptj, 1u2g, 2frd, 2oo5 – RrPTH domain I+III + NADP + NAD derivative
2oor – RrPTH domain I+III + NADP derivative + NAD
2bru – EcPTH domain I+III + NADP + NAD - NMR
4j16, 4j1t – PTH domain I+III + NADP + NAD – Thermus thermophilus
