4ol8

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Template:STRUCTURE 4ol8

Contents

Ty3 reverse transcriptase bound to DNA/RNA

Template:ABSTRACT PUBMED 24608367

Function

[YG31B_YEAST] Capsid protein (CA) is the structural component of the virus-like particle (VLP), forming the shell that encapsulates the genomic RNA-nucleocapsid complex.[1] [2] [3] [4] Nucleocapsid protein p11 (NC) forms the nucleocore that coats the retro-elements dimeric RNA. Binds these RNAs through its zinc fingers (By similarity). Promotes primer tRNA(i)-Met annealing to the multipartite primer-binding site (PBS), dimerization of Ty3 RNA and initiation of reverse transcription.[5] [6] [7] [8] The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.[9] [10] [11] [12] Reverse transcriptase/ribonuclease H (RT) is a multifunctional enzyme that catalyzes the conversion of the retro-elements RNA genome into dsDNA within the VLP. The enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes during plus-strand synthesis and hydrolyzes RNA primers. The conversion leads to a linear dsDNA copy of the retrotransposon that includes long terminal repeats (LTRs) at both ends.[13] [14] [15] [16] Integrase (IN) targets the VLP to the nucleus, where a subparticle preintegration complex (PIC) containing at least integrase and the newly synthesized dsDNA copy of the retrotransposon must transit the nuclear membrane. Once in the nucleus, integrase performs the integration of the dsDNA into the host genome.[17] [18] [19] [20]

About this Structure

4ol8 is a 8 chain structure. Full crystallographic information is available from OCA.

Reference

  • Nowak E, Miller JT, Bona MK, Studnicka J, Szczepanowski RH, Jurkowski J, Le Grice SF, Nowotny M. Ty3 reverse transcriptase complexed with an RNA-DNA hybrid shows structural and functional asymmetry. Nat Struct Mol Biol. 2014 Mar 9. doi: 10.1038/nsmb.2785. PMID:24608367 doi:http://dx.doi.org/10.1038/nsmb.2785
  1. ↑ Hansen LJ, Sandmeyer SB. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein. J Virol. 1990 Jun;64(6):2599-607. PMID:2159534
  2. ↑ Hansen LJ, Chalker DL, Orlinsky KJ, Sandmeyer SB. Ty3 GAG3 and POL3 genes encode the components of intracellular particles. J Virol. 1992 Mar;66(3):1414-24. PMID:1371165
  3. ↑ Orlinsky KJ, Sandmeyer SB. The Cys-His motif of Ty3 NC can be contributed by Gag3 or Gag3-Pol3 polyproteins. J Virol. 1994 Jul;68(7):4152-66. PMID:7515969
  4. ↑ Cristofari G, Gabus C, Ficheux D, Bona M, Le Grice SF, Darlix JL. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro. J Biol Chem. 1999 Dec 17;274(51):36643-8. PMID:10593967
  5. ↑ Hansen LJ, Sandmeyer SB. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein. J Virol. 1990 Jun;64(6):2599-607. PMID:2159534
  6. ↑ Hansen LJ, Chalker DL, Orlinsky KJ, Sandmeyer SB. Ty3 GAG3 and POL3 genes encode the components of intracellular particles. J Virol. 1992 Mar;66(3):1414-24. PMID:1371165
  7. ↑ Orlinsky KJ, Sandmeyer SB. The Cys-His motif of Ty3 NC can be contributed by Gag3 or Gag3-Pol3 polyproteins. J Virol. 1994 Jul;68(7):4152-66. PMID:7515969
  8. ↑ Cristofari G, Gabus C, Ficheux D, Bona M, Le Grice SF, Darlix JL. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro. J Biol Chem. 1999 Dec 17;274(51):36643-8. PMID:10593967
  9. ↑ Hansen LJ, Sandmeyer SB. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein. J Virol. 1990 Jun;64(6):2599-607. PMID:2159534
  10. ↑ Hansen LJ, Chalker DL, Orlinsky KJ, Sandmeyer SB. Ty3 GAG3 and POL3 genes encode the components of intracellular particles. J Virol. 1992 Mar;66(3):1414-24. PMID:1371165
  11. ↑ Orlinsky KJ, Sandmeyer SB. The Cys-His motif of Ty3 NC can be contributed by Gag3 or Gag3-Pol3 polyproteins. J Virol. 1994 Jul;68(7):4152-66. PMID:7515969
  12. ↑ Cristofari G, Gabus C, Ficheux D, Bona M, Le Grice SF, Darlix JL. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro. J Biol Chem. 1999 Dec 17;274(51):36643-8. PMID:10593967
  13. ↑ Hansen LJ, Sandmeyer SB. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein. J Virol. 1990 Jun;64(6):2599-607. PMID:2159534
  14. ↑ Hansen LJ, Chalker DL, Orlinsky KJ, Sandmeyer SB. Ty3 GAG3 and POL3 genes encode the components of intracellular particles. J Virol. 1992 Mar;66(3):1414-24. PMID:1371165
  15. ↑ Orlinsky KJ, Sandmeyer SB. The Cys-His motif of Ty3 NC can be contributed by Gag3 or Gag3-Pol3 polyproteins. J Virol. 1994 Jul;68(7):4152-66. PMID:7515969
  16. ↑ Cristofari G, Gabus C, Ficheux D, Bona M, Le Grice SF, Darlix JL. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro. J Biol Chem. 1999 Dec 17;274(51):36643-8. PMID:10593967
  17. ↑ Hansen LJ, Sandmeyer SB. Characterization of a transpositionally active Ty3 element and identification of the Ty3 integrase protein. J Virol. 1990 Jun;64(6):2599-607. PMID:2159534
  18. ↑ Hansen LJ, Chalker DL, Orlinsky KJ, Sandmeyer SB. Ty3 GAG3 and POL3 genes encode the components of intracellular particles. J Virol. 1992 Mar;66(3):1414-24. PMID:1371165
  19. ↑ Orlinsky KJ, Sandmeyer SB. The Cys-His motif of Ty3 NC can be contributed by Gag3 or Gag3-Pol3 polyproteins. J Virol. 1994 Jul;68(7):4152-66. PMID:7515969
  20. ↑ Cristofari G, Gabus C, Ficheux D, Bona M, Le Grice SF, Darlix JL. Characterization of active reverse transcriptase and nucleoprotein complexes of the yeast retrotransposon Ty3 in vitro. J Biol Chem. 1999 Dec 17;274(51):36643-8. PMID:10593967

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