1x8b

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Template:STRUCTURE 1x8b

Contents 1 Structure of human Wee1A kinase: kinase domain complexed with inhibitor PD0407824 2 Function 3 About this Structure 4 Reference

Structure of human Wee1A kinase: kinase domain complexed with inhibitor PD0407824

Template:ABSTRACT PUBMED 15837193

Function

[WEE1_HUMAN] Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.

About this Structure

1x8b is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

• Squire CJ, Dickson JM, Ivanovic I, Baker EN. Structure and inhibition of the human cell cycle checkpoint kinase, Wee1A kinase: an atypical tyrosine kinase with a key role in CDK1 regulation. Structure. 2005 Apr;13(4):541-50. PMID:15837193 doi:10.1016/j.str.2004.12.017