User:A. Rahim Zalal/Sandbox 1

From Proteopedia

Jump to: navigation, search

Histone (H2A)

H2A highlighted (green) in nucleosome. The complexed DNA is gray, and the rest of the core proteins are black. 1aoi
H2A highlighted (green) in nucleosome. The complexed DNA is gray, and the rest of the core proteins are black. 1aoi

The histone family's H2A protein family act as a subunits of eukaryotic nucleosomes. Specifically, H2A molecules form dimers with H2B molecules that interact with tetramers of histones H3 and H4 to form the octameric histone core of the nucleosome. All of the core histone proteins, including H2A, also interact with DNA in ways that allow for DNA compaction.

Contents


Background

The histone proteins are a family of proteins that allow for the compaction of DNA into functional units known as nucleosomes, which, through certain organized arrangements, form the chromatin fibers in eukaryotic organisms. The histone family includes the core histone families, H2A (shown highlighted on top right), H2B, H3, and H4, as well as the linker histone family, H1. The core histone proteins form the core of the nucleosome, while the linker histones sit on the outside of the nucleosome subunit and assist in interactions with other nucleosomes within the chromatin.


Structure

H2A:H2B dimer (H2A is represented in green while H2B is represented in gray), 1aoi
H2A:H2B dimer (H2A is represented in green while H2B is represented in gray), 1aoi

Function

Sequence

Protein Primary Sequence

The sequence for histone H2A type 1 (human) is presented here[1]: 

        10         20         30         40         50         60 
MSGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT 

        70         80         90        100        110        120 
AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK 

       130 
TESHHKAKGK

Coding DNA Sequence

The coding DNA sequence for the H2A type 1 subunit (human)[2]: 

ENA|AAA63191|AAA63191.1 Homo sapiens (human) histone H2A.1 : Location:1..393
ATGTCTGGACGTGGAAAGCAAGGCGGCAAAGCTCGGGCAAAAGCTAAAACGCGTTCTTCC
AGGGCCGGTCTTCAGTTTCCAGTTGGCCGTGTGCACCGCCTCCTCCGCAAAGGCAACTAC
TCCGAACGAGTCGGGGCCGGCGCTCCAGTGTACCTGGCAGCGGTGCTGGAATATCTGACG
GCCGAGATCTTAGAGCTAGCTGGCAACGCGGCTCGCGACAATAAGAAGACCCGCATCATC
CCGCGCCACCTGCAGCTAGCCATCCGCAACGACGAGGAGCTAAATAAGCTTCTAGGTCGC
GTGACCATCGCGCAGGGCGGTGTCCTGCCCAACATCCAGGCCGTATTGCTGCCTAAGAAG
ACGGAGAGCCACCATAAGGCCAAGGGCAAGTGA

Mechanism

This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.


References

  1. ↑ Marzluff WF, Gongidi P, Woods KR, Jin J, Maltais LJ. The human and mouse replication-dependent histone genes. Genomics. 2002 Nov;80(5):487-98. PMID:12408966
  2. ↑ Albig W, Kardalinou E, Drabent B, Zimmer A, Doenecke D. Isolation and characterization of two human H1 histone genes within clusters of core histone genes. Genomics. 1991 Aug;10(4):940-8. PMID:1916825

Proteopedia Page Contributors and Editors (what is this?)

A. Rahim Zalal

Retrieved from "http://52.214.119.220/wiki/index.php/User:A._Rahim_Zalal/Sandbox_1"
Personal tools