Publication Abstract from PubMed
Ruminococcus flavefaciens is a cellulolytic bacterium found in the rumen of herbivores and produces one of the most elaborate and variable cellulosome systems. The structure of an R. flavefaciens protein (RfCohG, ZP_06142108), representing a freestanding (non-cellulosomal) type III cohesin module, has been determined. A selenomethionine derivative with a C-terminal histidine tag was crystallized and diffraction data were measured to 2.44 A resolution. Its structure was determined by single-wavelength anomalous dispersion, revealing eight molecules in the asymmetric unit. RfCohG exhibits the most complex among all known cohesin structures, possessing four alpha-helical elements and a topographical protuberance on the putative dockerin-binding surface.
Structural characterization of a novel autonomous cohesin from Ruminococcus flavefaciens.,Voronov-Goldman M, Levy-Assaraf M, Yaniv O, Wisserman G, Jindou S, Borovok I, Bayer EA, Lamed R, Shimon LJ, Frolow F Acta Crystallogr F Struct Biol Commun. 2014 Apr;70(Pt 4):450-6. doi:, 10.1107/S2053230X14004051. Epub 2014 Mar 25. PMID:24699736[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
