Overview
The native E. coli BamB structure contains residues 21–392 arranged as an eight-bladed β-propeller with hole at the center. Each blade is connected by interconnected loop (IL). BamB is strongly electronegative at the center at either side of the hole due to the presence of strong electronegative residues in these regions (E197, D246, D248, D288, D303, E370). Among the ILs, IL4 and IL5 are unordered and the interacting residues are found in these loops. How the overall structure looks like? Subunits orientation, chains: the number and relative positions, etc.
Here is the
of the main chain of lysozyme. And here is the of the same lysozyme molecule.
Interactions with other molecules
BamB interact with BamA through residues L192, L194, R195, D246, and D248. These residues are found on IL4 ( L192, L194, R195) and IL5 (D246, D248). BamB interacts with the PORTA 2-4 regions of BamA. The IL4 of the The BamB (R195)protein interact with the PORTA 3 regions of the BamA (D241)region by forming a salt bridge. BamB form a triple complex: BamB-BamA-SurA and mediate the interaction between BamA and SurA and facilitate the delivery, folding and insertion of OMPs into the outer membrane. interact with the Close up picture, with explanation what we see
Active site organisation
The active site of BamB are found in IL4 (L192, L194, R195) and IL5(D246, and D248).
Conclusions
