3s5x is a 1 chain structure with sequence from Planktothrix agardhii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
The cyanobacterial Oscillatory Agardhii agglutinin (OAA) is a recently discovered HIV-inactivating lectin that interacts with high-mannose sugars. Nuclear magnetic resonance (NMR) binding studies between OAA and alpha3,alpha6-mannopentaose (Manalpha(1-3)[Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)]Man), the branched core unit of Man-9, revealed two binding sites at opposite ends of the protein, exhibiting essentially identical affinities. Atomic details of the specific protein-sugar contacts in the recognition loops of OAA were delineated in the high-resolution crystal structures of free and glycan-complexed protein. No major changes in the overall protein structure are induced by carbohydrate binding, with essentially identical apo- and sugar-bound conformations in binding site 1. A single peptide bond flip at W77-G78 is seen in binding site 2. Our combined NMR and crystallographic results provide structural insights into the mechanism by which OAA specifically recognizes the branched Man-9 core, distinctly different from the recognition of the D1 and D3 arms at the nonreducing end of high-mannose carbohydrates by other antiviral lectins.
Structural basis of the anti-HIV activity of the cyanobacterial Oscillatoria Agardhii agglutinin.,Koharudin LM, Gronenborn AM Structure. 2011 Aug 10;19(8):1170-81. PMID:21827952[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Koharudin LM, Gronenborn AM. Structural basis of the anti-HIV activity of the cyanobacterial Oscillatoria Agardhii agglutinin. Structure. 2011 Aug 10;19(8):1170-81. PMID:21827952 doi:10.1016/j.str.2011.05.010
