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3sbm is a 1 chain structure with sequence from Sorangium cellulosum. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
The 1.51 A resolution X-ray crystal structure of the trans-acyltransferase (AT) from the "AT-less" disorazole synthase (DSZS) and that of its acetate complex at 1.35 A resolution are reported. Separately, comprehensive alanine-scanning mutagenesis of one of its acyl carrier protein substrates (ACP1 from DSZS) led to the identification of a conserved Asp45 residue on the ACP, which contributes to the substrate specificity of this unusual enzyme. Together, these experimental findings were used to derive a model for the selective association of the DSZS AT and its ACP substrate. With a goal of structurally characterizing the AT-ACP interface, a strategy was developed for covalently cross-linking the active site Ser --> Cys mutant of the DSZS AT to its ACP substrate and for purifying the resulting AT-ACP complex to homogeneity. The S86C DSZS AT mutant was found to be functional, albeit with a transacylation efficiency 200-fold lower than that of its wild-type counterpart. Our findings provide new insights as well as new opportunities for high-resolution analysis of an important protein-protein interface in polyketide synthases.
Structure and Mechanism of the trans-Acting Acyltransferase from the Disorazole Synthase.,Wong FT, Jin X, Mathews II, Cane DE, Khosla C Biochemistry. 2011 Jul 5. PMID:21707057[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Wong FT, Jin X, Mathews II, Cane DE, Khosla C. Structure and Mechanism of the trans-Acting Acyltransferase from the Disorazole Synthase. Biochemistry. 2011 Jul 5. PMID:21707057 doi:10.1021/bi200632j
