Publication Abstract from PubMed
PDZRhoGEF (PRG) belongs to a small family of RhoA-specific nucleotide exchange factors, which mediate signaling through select G-protein coupled receptors (GPCRs) via Galpha12/13, and activate RhoA by catalyzing the exchange of GDP to GTP. PRG is a multidomain protein composed of PDZ, RGSL, DH and PH domains. It is autoinhibited in cytosol, and is believed to undergo a conformational rearrangement and translocation to the membrane for full activation, although the molecular details of the regulation mechanism are not clear. It has been shown recently, that the main autoregulatory elements of PDZRhoGEF - the autoinhibitory 'activation box' and the 'GEF switch', which is required for full activation, are located directly upstream of the catalytic DH domain and its RhoA-binding surface, emphasizing the functional role of the RGSL-DH linker. Here, using a combination of biophysical and biochemical methods, we show that the mechanism of PRG regulation is yet more complex, and may involve an additional autoinhibitory element in the form of a molten globule region within the linker between RGSL and DH domains. We propose a novel, two-tier model of autoinhibition, where the 'activation box' and the molten globule region act synergistically to impair the ability of RhoA to bind to the catalytic DH-PH tandem. The molten globule region and the 'activation box' become less ordered in the PRG/RhoA complex, and dissociate from the RhoA binding site, which may constitute a critical step leading to PRG activation.
Insights into the molecular activation mechanism of the RhoA-specific guanine nucleotide exchange factor, PDZRhoGEF.,Bielnicki JA, Shkumatov A, Derewenda U, Somlyo AV, Svergun DI, Derewenda ZS J Biol Chem. 2011 Aug 4. PMID:21816819[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
