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3zqw is a 1 chain structure with sequence from Acetivibrio cellulolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
The carbohydrate-binding module (CBM) of the major scaffoldin subunit ScaA of the cellulosome of Acetivibrio cellulolyticus is classified as a family 3b CBM and binds strongly to cellulose. The CBM3b was overexpressed, purified and crystallized, and its three-dimensional structure was determined. The structure contained a nickel-binding site located at the N-terminal region in addition to a 'classical' CBM3b calcium-binding site. The structure was also determined independently by the SAD method using data collected at the Ni-absorption wavelength of 1.48395 A and even at a wavelength of 0.97625 A in a favourable case. The new scaffoldin-borne CBM3 structure reported here provides clear evidence for the proposition that a family 3b CBM may be accommodated in scaffoldin subunits and functions as the major substrate-binding entity of the cellulosome assembly.
Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA from Acetivibrio cellulolyticus.,Yaniv O, Halfon Y, Shimon LJ, Bayer EA, Lamed R, Frolow F Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):8-13. Epub , 2011 Dec 24. PMID:22232162[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Yaniv O, Halfon Y, Shimon LJ, Bayer EA, Lamed R, Frolow F. Structure of CBM3b of the major cellulosomal scaffoldin subunit ScaA from Acetivibrio cellulolyticus. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jan 1;68(Pt 1):8-13. Epub , 2011 Dec 24. PMID:22232162 doi:10.1107/S174430911104807X
