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3zy7 is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Computational design of novel protein-protein interfaces is a test of our understanding of protein interactions and has the potential to allow modification of cellular physiology. Methods for designing high-affinity interactions that adopt a predetermined binding mode have proved elusive, suggesting the need for new strategies that simplify the design process. A solvent-exposed backbone on a beta-strand is thought of as "sticky" and beta-strand pairing stabilizes many naturally occurring protein complexes. Here, we computationally redesign a monomeric protein to form a symmetric homodimer by pairing exposed beta-strands to form an intermolecular beta-sheet. A crystal structure of the designed complex closely matches the computational model (rmsd = 1.0 A). This work demonstrates that beta-strand pairing can be used to computationally design new interactions with high accuracy.
Computational design of a symmetric homodimer using beta-strand assembly.,Stranges PB, Machius M, Miley MJ, Tripathy A, Kuhlman B Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20562-7. Epub 2011 Dec 5. PMID:22143762[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Stranges PB, Machius M, Miley MJ, Tripathy A, Kuhlman B. Computational design of a symmetric homodimer using beta-strand assembly. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20562-7. Epub 2011 Dec 5. PMID:22143762 doi:10.1073/pnas.1115124108
