2ca9
From Proteopedia
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APO-NIKR FROM HELICOBACTER PYLORI IN CLOSED TRANS-CONFORMATION
Overview
The survival of Helicobacter pylori in the human stomach critically relies, on the availability and use of nickel, an absolute cofactor of the, important virulence determinant urease. Nickel-responsive gene regulation, is mediated by HpNikR, a protein belonging to the ribbon-helix-helix, family of transcriptional regulators. Unlike its homologues, HpNikR acts, as both a repressor and an activator within an acid adaptation cascade. We, report the crystal structure of the full-length HpNikR in a nickel-free, conformation and two nickel-bound structures obtained in different, conditions: Ni1-HpNikR and Ni2-HpNikR. Apo-HpNikR shows the same global, fold as its bacterial homologues although with an unusual closed, trans-conformation and asymmetrical quaternary arrangement. The structure, of ... [(full description)]
About this Structure
2CA9 is a [Single protein] structure of sequence from [Helicobacter pylori] with FMT and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Structural basis of the nickel response in Helicobacter pylori: crystal structures of HpNikR in Apo and nickel-bound states., Dian C, Schauer K, Kapp U, McSweeney SM, Labigne A, Terradot L, J Mol Biol. 2006 Aug 25;361(4):715-30. Epub 2006 Jul 7. PMID:16872629
Page seeded by OCA on Tue Oct 30 17:03:11 2007
Categories: Helicobacter pylori | Single protein | Dian, C. | Kapp, U. | Labigne, A. | Mcsweeney, S.M. | Schauer, K. | Terradot, L. | FMT | GOL | Acidic-adaptive response | Dna-binding | Hypothetical protein | Metal-binding | Nickel | Nickel uptake | Ribbon-helix-helix | Transcription regulator | Transcriptional regulation
