3va1 is a 2 chain structure with sequence from Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Mammalian MDC1 interacts with CHK2 in the regulation of DNA damage-induced S-phase checkpoint and apoptosis, which is directed by the association of MDC1-FHA and CHK2-pThr68. However, different ligand specificities of MDC1-FHA have been reported, and no structure is available. Here we report the crystal structures of MDC1-FHA and its complex with a CHK2 peptide containing pThr68. Unlike other FHA domains, MDC1-FHA exists as an intrinsic dimer in solution and in crystals. Structural and binding analyses support pThr+3 ligand specificity and provide structural insight into MDC1-CHK2 interaction.
Structural Delineation of MDC1-FHA Domain Binding with CHK2-pThr68.,Wu HH, Wu PY, Huang KF, Kao YY, Tsai MD Biochemistry. 2012 Jan 17;51(2):575-7. Epub 2012 Jan 6. PMID:22211259[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Wu HH, Wu PY, Huang KF, Kao YY, Tsai MD. Structural Delineation of MDC1-FHA Domain Binding with CHK2-pThr68. Biochemistry. 2012 Jan 17;51(2):575-7. Epub 2012 Jan 6. PMID:22211259 doi:10.1021/bi201709w
