1bvk
From Proteopedia
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| , resolution 2.7Å | |||||||
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| Activity: | Lysozyme, with EC number 3.2.1.17 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HUMANIZED ANTI-LYSOZYME FV COMPLEXED WITH LYSOZYME
Overview
The crystal structure of the complex between hen egg lysozyme and the Fv fragment of a humanized antilysozyme antibody was determined to 2.7-A resolution. The structure of the antigen combining site in the complex is nearly identical to that of the complexed form of the parent mouse antibody, D1.3. In contrast, the combining sites of the unliganded mouse and humanized antilysozymes show moderate conformational differences. This disparity suggests that a conformational readjustment process linked to antigen binding reverses adverse conformations in the complementarity determining regions that had been introduced by engineering these segments next to human framework regions in the humanized antibody.
About this Structure
1BVK is a Protein complex structure of sequences from Gallus gallus and Homo sapiens. Full crystallographic information is available from OCA.
Reference
Conformational correction mechanisms aiding antigen recognition by a humanized antibody., Holmes MA, Buss TN, Foote J, J Exp Med. 1998 Feb 16;187(4):479-85. PMID:9463398
Page seeded by OCA on Thu Mar 20 10:16:18 2008
