| Structural highlights
4ar7 is a 1 chain structure with sequence from Aequorea victoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | NonStd Res: | |
| Related: | 1b9c, 1bfp, 1c4f, 1cv7, 1ema, 1emb, 1emc, 1eme, 1emf, 1emg, 1emk, 1eml, 1emm, 1f09, 1f0b, 1gfl, 1h6r, 1hcj, 1huy, 1jby, 1jbz, 1jc0, 1jc1, 1kp5, 1kyp, 1kyr, 1kys, 1myw, 1q4a, 1q4b, 1q4c, 1q4d, 1q4e, 1q73, 1qxt, 1qy3, 1qyf, 1qyo, 1qyq, 1rm9, 1rmm, 1rmo, 1rmp, 1rrx, 1w7s, 1w7t, 1w7u, 1yfp, 1yhg, 1yhh, 1yhi, 1yj2, 1yjf, 1z1p, 1z1q, 2ah8, 2aha, 2b3p, 2b3q, 2emd, 2emn, 2emo, 2fwq, 2fzu, 2wsn, 2wso, 2wur, 2y0g, 2ydz, 2ye0, 2ye1, 2yfp, 3ztf, 4anj, 4as8 |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Publication Abstract from PubMed
The green fluorescent protein (GFP) from the jellyfish Aequoria victoria has been shown to dimerize at high concentrations, which could lead to artefacts in imaging experiments. To ensure a truly monomeric state, an A206K mutation has been introduced into most of its widely used variants, with minimal effect on the spectroscopic properties. Here, the first structure of one of these variants, the cyan fluorescent protein mTurquoise, is presented and compared with that of its dimeric version mTurquoise-K206A. No significant structural change is detected in the chromophore cavity, reinforcing the notion that this mutation is spectroscopically silent and validating that the structural analysis performed on dimeric mutants also applies to monomeric versions. Finally, it is explained why cyan versions of GFP containing the Y66W and N146I mutations do not require the A206K mutation to prevent dimerization at high concentrations.
Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K.,von Stetten D, Noirclerc-Savoye M, Goedhart J, Gadella TW Jr, Royant A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug 1;68(Pt 8):878-82., Epub 2012 Jul 26. PMID:22869113[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ von Stetten D, Noirclerc-Savoye M, Goedhart J, Gadella TW Jr, Royant A. Structure of a fluorescent protein from Aequorea victoria bearing the obligate-monomer mutation A206K. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug 1;68(Pt 8):878-82., Epub 2012 Jul 26. PMID:22869113 doi:10.1107/S1744309112028667
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