Carboxypeptidase A catalyses the release of the C-terminal amino acid from a polypeptide chain. The initial image shows carboxypeptidase with a tetrapeptide (green) bound in the active site.
The enzyme has . A is present. The ion is involved in binding and catalysis.
Substrate binding involves three interactions between the substrate and the active site.
1. .
2. .
3. .
Catalysis involves .
(The tetrapeptide is actually the C-terminus of a protein that inhibits carboxypeptidase A. This portion of the inhibitor mimics the binding of substrate to the active site.)
