2cdb

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 2cdb, resolution 1.600Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

SULFOLOBUS SOLFATARICUS GLUCOSE DEHYDROGENASE 1 IN COMPLEX WITH NADP AND GLUCOSE

Overview

The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally, above 80 degrees C and utilizes an unusual, promiscuous, non-phosphorylative Entner-Doudoroff pathway to metabolize both glucose, and galactose. The first enzyme in this pathway, glucose dehydrogenase, catalyzes the oxidation of glucose to gluconate, but has been shown to, have activity with a broad range of sugar substrates, including glucose, galactose, xylose, and L-arabinose, with a requirement for the glucose, stereo configuration at the C2 and C3 positions. Here we report the, crystal structure of the apo form of glucose dehydrogenase to a resolution, of 1.8 A and a complex with its required cofactor, NADP+, to a resolution, of 2.3 A. A T41A mutation was engineered to enable the trapping of, substrate in the ... [(full description)]

About this Structure

2CDB is a [Single protein] structure of sequence from [Sulfolobus solfataricus] with BGC, ZN, EDO and NAP as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

Reference

The structural basis of substrate promiscuity in glucose dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus., Milburn CC, Lamble HJ, Theodossis A, Bull SD, Hough DW, Danson MJ, Taylor GL, J Biol Chem. 2006 May 26;281(21):14796-804. Epub 2006 Mar 23. PMID:16556607

Page seeded by OCA on Tue Oct 30 17:05:10 2007