Dehalogenase

Function

Dehalogenases catalyze the removal of halogen atom from substrates.
• Haloalkane dehalogenase converts haloalkanes to alcohol
• Halohydrin dehalogenase converts halohydrins to epoxide
• L-2 haloacid dehalogenase converts haloacids to hydroxyacid
• 4-chlorobenzoyl CoA dehalogenase converts 4-chlorobenzoyl CoA to 4-hydroxybenzoyl CoA
• Fluoroacetate dehalogenase converts fluoroacetate to glycolate
• Iodotyrosine dehalogenase converts iodotyrosine to tyrosine
• cis- and trans-3-chloroacrylic acid dehalogenase converts 3-chloroacrylate to malonate semialdehyde
• 5-chloromuconolactone dehalogenase converts 5-chloromuconolactone to E-dienelactone.

Disease

Relevance

Structural highlights

3D structures of dehalogenase

Updated on 16-July-2014

Haloalkane dehalogenase (HDH)

2had, 1ede, 2pky, 2yxp – XaHDH – Xanthobacter autotrophicus
1hde, 1be0, 1bee, 1bez – XaHDH (mutant)
1bn6, 1bn7, 4kaa, 4kac, 4kaf – RrHDH – Rhodococcus rhodochrous
2v9z – RrHDH (mutant)
1cv2 – SpHDH – Sphingomonas paucimobilis
4h77 – SphHDH – Sphignobium
4h7d – SphHDH (mutant)

Haloalkane dehalogenase complex with halide ion

1edb, 1edd, 2eda, 2edc, 2dhe, 1b6g – XaHDH + halide

1cij – XaHDH (mutant) + halide
1d07, 1iz7, 1k5p, 1mj5 – SpHDH + halide
3fbw, 1cqw, 4hzg – RrHDH + halide
3a2m, 3a2n, 3afi – BjHDH + halide – Bradyrhizobium japonicum
3a2l – BjHDH (mutant) + halide
4k2a – HDH + halide – Bradyrhizobium elkanii
3rk4, 3sk0, 4f5z, 4f60 – RrHDH (mutant) + halide
4h7e, 4h7f, 4h7h, 4h7i, 4h7j 4h7k – SphHDH (mutant) + halide
4brz – RhHDH + halide - Rhodobacteraceae

Haloalkane dehalogenase other complexes

2dhc – XaHDH + ethylene dichloride

2dhd – XaHDH + amono-chloroethoxy-oxobutanoic acid + Cl
1g42 – SpHDH + dichloropropane + Cl
2bfn – SpHDH + dichloropropanol + Cl
1g4h – SpHDH (mutant) + butanol + Cl
1g5f – SpHDH + dichloroethane + Cl
1iz8 – SpHDH + propanediol + Cl
4c6h – RhHDH + hexanol + Cl
3fwh, 3g9x, 4e46 – RrHDH + isopropanol + Cl
1k6e – SpHDH + bromopropanol + Cl + Br
1k63 – SpHDH + bromopropene-1-ol + Cl + Br
4fwb – SpHDH (mutant) + trichloropropane + Cl

Fluoroacetate dehalogenase (FDH)

1y37 – BuFDH – Burkholderia
3r3u – RpFDH – Rhodopseudomonas palustris
3r40, 3r41 – RpFDH (mutant)

Fluoroacetate dehalogenase complexes

3r3v, 3r3y – RpFDH (mutant) + fluoroacetate

3r3w – RpFDH (mutant) + chloroacetate
3r3x – RpFDH (mutant) + bromoacetate
3r3z – RpFDH + glycolate
3b12 – BuFDH (mutant) + fluoroacetate

Halohydrin dehalogenase (HHDH)

1zmo – HHDH – Anthrobacter
3zn2 – AtHHDH – Agrobacterium tumefaciens

Halohydrin dehalogenase complexes

1pwx – AtHHDH + Br

1px0 – AtHHDH + haloalcohol mimic
1zo8 – AtHHDH + p-nitrostyrene oxide
4ixt – AtHHDH (mutant) + ethyl cyano-hydroxybutyrate
4ixw – RrHHDH (mutant) + ethyl oxiranyl acetate – Rhizobium radiobacter
4iy1 – RrHHDH + Cl

L-2-haloacid dehalogenase (LHDH)

1jud – PsLHDH – Pseudomonas
2w43 – StLHDH – Sulfolobus tokodaii

L-2-haloacid dehalogenase complexes

1aq6, 1qq5 – XaLHDH + formic acid

1zrm – PsLHDH (mutant) + butanoic acid
1zrn – PsLHDH (mutant) + acetic acid
1qh9 – PsLHDH + lactic acid
2w11 – StLHDH + lactic acid
1qq6, 1qq7 – XaLHDH + aspartate carboxymethyl ester + Cl

DL-2-haloacid dehalogenase (DLHDH)

4n2x – MeDLHDH – Methylobacterium
3wj8 – MeDLHDH + bromo-methylpropionate

Iodotyrosine dehalogenase (ITDH)

3gb5 – mITDH – mouse
3to0 – mITDH (mutant)
3gfd – mITDH + iodotyrosine
3tnz – mITDH (mutant) + iodotyrosine
3gh8 – mITDH + diiodotyrosine

cis-3-chloroacrylic acid dehalogenase (CaaD)

2flz, 3mf8 – CbCaaD – Coryneform bacterium
3mf7 – CbCaaD + oxirane-carboxylate
2flt – CbCaaD + lactic acid

trans-3-chloroacrylic acid dehalogenase (TaaD)

3ej3 – PpTaaD α+β subunits – Pseudomonas pavonacea
3ej7, 3ej9 – PpTaaD α (mutant) +β subunits
1s0y – PpTaaD α+β subunits + malonic acid

5-chloromuconolactone dehalogenase (ClcF)

3znu, 3znj, 4fpi – RoClcF – Rhodococcus opacus
3zo7 – RoClcF + 5-chloromuconolactone

4-chlorobenzoyl CoA dehalogenase (CBCD)

1nzy – PsCBCD
1jxz – PsCBCD + hydroxybenzoyl CoA

References