Publication Abstract from PubMed
Conjugative plasmid transfer is the most important route for the spread of resistance and virulence genes among bacteria. Consequently, bacteria carrying conjugative plasmids are a substantial threat to human health, especially hospitalized patients. Whilst detailed information about the process has been obtained for Gram-negative type-4 secretion systems, little is known about the corresponding mechanisms in Gram-positive (G+) bacteria. The successful purification and crystallization of the putative transfer protein TraN from the G+ conjugative model plasmid pIP501 of Enterococcus faecalis are presented. Native crystals diffracted to 1.8 A resolution on a synchrotron beamline. The crystals belonged to space group P2(1), with unit-cell parameters a=32.88, b=54.94, c=57.71 A, beta=91.89 degrees and two molecules per asymmetric unit.
Crystallization and first data collection of the putative transfer protein TraN from the Gram-positive conjugative plasmid pIP501.,Goessweiner-Mohr N, Fercher C, Abajy MY, Grohmann E, Keller W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1402-5., doi: 10.1107/S174430911204184X. Epub 2012 Oct 30. PMID:23143259[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
