Protein kinase C

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Template:STRUCTURE 3gpe

Protein kinase C (PKC) phosphorylate serine or threonine residues in proteins. They act in signal transduction pathways. Conventional PKC (CPKC) - α, β1, β2, γ – are activated by diacylglycerol (DAG), Ca+2 and a phospholipid. Novel PKC (NPKC) – δ, ε, η, θ – are activated by DAG. Atypical (APKC) do not require DAG or Ca+2 for activation. PKC consists of regulatory domain hinged to a catalytic domain. The regulatory domain contains the C1 region which binds DAG and phorbol esters and the C2 domain which is a Ca+2 sensor. PKC contains Pleckstrin Homology (PH) domain which binds phosphatidylinositol lipids (PTDINS). The PH domain is found in proteins involved in intracellular signaling.

Contents 1 3D structures of protein kinase C 2 Conventional PKCs 2.1 PKC-a 2.2 PKC-β 2.3 PKC-β2 2.4 PKC-γ 3 Novel PKC 3.1 PKC-δ 3.2 PKC-ε 3.3 PKC-τ 3.4 PKC-η 3.5 PKC-θ 4 Atypical PKC 4.1 PKC-ι 4.2 PKC-ν

3D structures of protein kinase C

Updated on 20-August-2014

Conventional PKCs

PKC-a

1dsy, 3rdj, 3twy – rCPKC-α C2 domain – rat

3rdl, 4l1l - rCPKC-α C2 domain + ion

3gpe - rCPKC-α C2 domain + Ca + PTDINS

2eli – hCPKC-α C1 domain – human – NMR
4dnl - hCPKC-α C2 domain
3iw4 - hCPKC-α kinase domain + inhibitor

PKC-β

1a25 – rCPKC-β C2 domain

PKC-β2

2i0e - hCPKC-β2 catalytic domain

3pfq - rCPKC-β2 (mutant)

PKC-γ

2uzp - hCPKC-γ C2 domain

2e73 - hCPKC-γ C1 domain - NMR

1tbn, 1tbo - rCPKC-γ C2 domain – NMR

Novel PKC

PKC-δ

1ptq – mNPKC-δ C2 domain – mouse
3uej - mNPKC-δ C1B domain
3uey, 3uff, 3ugd, 3ugi, 3ugl - mNPKC-δ C1B domain (mutant)

1ptr - mNPKC-δ C2 domain + phorbol-acetate

1bdy - rNPKC-δ C2 domain

1yrk – hNPKC-δ C2 domain + peptide

2yuu - hNPKC-δ C1 domain - NMR

2coa - hNPKC-δ PH domain – NMR

PKC-ε

1gmi – rNPKC-ε C2 domain

PKC-τ

2enj - hNPKC-τ C2 domain – NMR

2enn, 2enz - hNPKC-τ C1 domain – NMR

1xjd – hNPKC-τ + saurosporine

2jed - hNPKC-τ kinase domain + inhibitor

PKC-η

2fk9 – hNPKC-η C2 domain
3txo - hNPKC-η kinase domain (mutant) + inhibitor

PKC-θ

4fkd – hNPKC-θ second Cys-rich regulatory domain

Atypical PKC

PKC-ι

1vd2 – hAPKC-ι PB1 domain – NMR

1zrz - hAPKC-ι catalytic domain

3a8w, 3a8x - hAPKC-ι kinase domain
3zh8 - hAPKC-ι kinase domain (mutant) + inhibitor
1wmh - hAPKC-ι PB1 domain + PAR6 alpha
4dc2 - hAPKC-ι residues 231-595 + PAR3 peptide

PKC-ν

2d9z – hPKC-ν PH domain - NMR