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1cy9
From Proteopedia
Revision as of 09:27, 27 August 2014 by OCA (Talk | contribs)
1cy9 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
DNA topoisomerases are the enzymes responsible for maintaining the topological states of DNA. In order to change the topology of DNA, topoisomerases pass one or two DNA strands through transient single or double strand breaks in the DNA phosphodiester backbone. It has been proposed that both type IA and type II enzymes change conformation dramatically during the reaction cycle in order to accomplish these transformations. In the case of Escherichia coli DNA topoisomerase I, it has been suggested that a 30 kDa fragment moves away from the rest of the protein to create an entrance into the central hole in the protein. Structures of the 30 kDa fragment reveal that indeed this fragment can change conformation significantly. The fragment is composed of two domains, and while the domains themselves remain largely unchanged, their relative arrangement can change dramatically.
Conformational changes in E. coli DNA topoisomerase I.,Feinberg H, Lima CD, Mondragon A Nat Struct Biol. 1999 Oct;6(10):918-22. PMID:10504724[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Feinberg H, Lima CD, Mondragon A. Conformational changes in E. coli DNA topoisomerase I. Nat Struct Biol. 1999 Oct;6(10):918-22. PMID:10504724 doi:10.1038/13283
