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1eg7
From Proteopedia
Revision as of 11:20, 24 September 2014 by OCA (Talk | contribs)
1eg7 is a 2 chain structure with sequence from Moorella thermoacetica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The structure was solved at 2.5 A resolution using multiwavelength anomalous dispersion (MAD) scattering by Se-Met residues. The subunit of N(10)-formyltetrahydrofolate synthetase is composed of three domains organized around three mixed beta-sheets. There are two cavities between adjacent domains. One of them was identified as the nucleotide binding site by homology modeling. The large domain contains a seven-stranded beta-sheet surrounded by helices on both sides. The second domain contains a five-stranded beta-sheet with two alpha-helices packed on one side while the other two are a wall of the active site cavity. The third domain contains a four-stranded beta-sheet forming a half-barrel. The concave side is covered by two helices while the convex side is another wall of the large cavity. Arg 97 is likely involved in formyl phosphate binding. The tetrameric molecule is relatively flat with the shape of the letter X, and the active sites are located at the end of the subunits far from the subunit interface.
The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica.,Radfar R, Shin R, Sheldrick GM, Minor W, Lovell CR, Odom JD, Dunlap RB, Lebioda L Biochemistry. 2000 Apr 11;39(14):3920-6. PMID:10747779[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Radfar R, Shin R, Sheldrick GM, Minor W, Lovell CR, Odom JD, Dunlap RB, Lebioda L. The crystal structure of N(10)-formyltetrahydrofolate synthetase from Moorella thermoacetica. Biochemistry. 2000 Apr 11;39(14):3920-6. PMID:10747779
