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1iot
From Proteopedia
Revision as of 10:08, 28 September 2014 by OCA (Talk | contribs)
1iot is a 1 chain structure with sequence from Gallus gallus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Stabilization of a protein using cavity-filling strategy has hardly been successful because of unfavorable van der Waals contacts. We succeeded in stabilizing lysozymes by cavity-filling mutations. The mutations were checked by a simple energy minimization in advance. It was shown clearly that the sum of free energy change caused by the hydrophobicity and the cavity size was correlated very well with protein stability. We also considered the aromatic-aromatic interaction. It is reconfirmed that the cavity-filling mutation in a hydrophobic core is a very useful method to stabilize a protein when the mutation candidate is selected carefully.
Stabilization of hen egg white lysozyme by a cavity-filling mutation.,Ohmura T, Ueda T, Ootsuka K, Saito M, Imoto T Protein Sci. 2001 Feb;10(2):313-20. PMID:11266617[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Ohmura T, Ueda T, Ootsuka K, Saito M, Imoto T. Stabilization of hen egg white lysozyme by a cavity-filling mutation. Protein Sci. 2001 Feb;10(2):313-20. PMID:11266617
