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1f36
From Proteopedia
Revision as of 12:58, 28 September 2014 by OCA (Talk | contribs)
1f36 is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Fis protein regulates site-specific DNA inversion catalyzed by a family of DNA invertases when bound to a cis-acting recombinational enhancer. As is often found for transactivation domains, previous crystal structures have failed to resolve the conformation of the N-terminal inversion activation region within the Fis dimer. A new crystal form of a mutant Fis protein now reveals that the activation region contains two beta-hairpin arms that protrude over 20 A from the protein core. Saturation mutagenesis identified the regulatory and structurally important amino acids. The most critical activating residues are located near the tips of the beta-arms. Disulfide cross-linking between the beta-arms demonstrated that they are highly flexible in solution and that efficient inversion activation can occur when the beta-arms are covalently linked together. The emerging picture for this regulatory motif is that contacts with the recombinase at the tip of the mobile beta-arms activate the DNA invertase in the context of an invertasome complex.
The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms.,Safo MK, Yang WZ, Corselli L, Cramton SE, Yuan HS, Johnson RC EMBO J. 1997 Nov 17;16(22):6860-73. PMID:9362499[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Safo MK, Yang WZ, Corselli L, Cramton SE, Yuan HS, Johnson RC. The transactivation region of the fis protein that controls site-specific DNA inversion contains extended mobile beta-hairpin arms. EMBO J. 1997 Nov 17;16(22):6860-73. PMID:9362499 doi:10.1093/emboj/16.22.6860
