This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1om9
From Proteopedia
Revision as of 14:08, 28 September 2014 by OCA (Talk | contribs)
1om9 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Golgi-associated, gamma-adaptin-related, ADP-ribosylation-factor binding proteins (GGAs) and adaptor protein (AP)-1 are adaptors involved in clathrin-mediated transport between the trans-Golgi network and endosomal system. The appendage domains of GGAs and the AP-1 gamma-adaptin subunit are structurally homologous and have been proposed to bind to accessory proteins via interaction with short sequences containing phenylalanines and acidic residues. Here we present the structure of the human GGA1 appendage in complex with its cognate binding peptide from the p56 accessory protein (DDDDFGGFEAAETFD) as determined by X-ray crystallography. The interaction is governed predominantly by packing of the first two phenylalanine residues of the peptide with conserved basic and hydrophobic residues from GGA1. Additionally, several main chain hydrogen bonds cause the peptide to form an additional beta-strand on the edge of the preexisting beta-sheet of the protein. Isothermal titration calorimetry was used to assess the affinities of different peptides for the GGA and gamma-appendage domains.
Structural basis for binding of accessory proteins by the appendage domain of GGAs.,Collins BM, Praefcke GJ, Robinson MS, Owen DJ Nat Struct Biol. 2003 Aug;10(8):607-13. PMID:12858163[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Collins BM, Praefcke GJ, Robinson MS, Owen DJ. Structural basis for binding of accessory proteins by the appendage domain of GGAs. Nat Struct Biol. 2003 Aug;10(8):607-13. PMID:12858163 doi:10.1038/nsb955
