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1nhc
From Proteopedia
Revision as of 15:28, 28 September 2014 by OCA (Talk | contribs)
1nhc is a 6 chain structure with sequence from Aspergillus niger. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Endopolygalacturonase I is a processive enzyme, while the 60% sequence identical endopolygalacturonase II is not. The 1.70 A resolution crystal structure of endopolygalacturonase I reveals a narrowed substrate binding cleft. In addition, Arg96, a residue in this cleft previously shown to be critical for processivity, interacts with the substrate mimics glycerol and sulfate in several well-defined conformations in the six molecules in the asymmetric unit. From this we conclude that both Arg96 and the narrowed substrate binding cleft contribute to retaining the substrate while it moves through the active site after a cleavage event has occurred.
Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger.,van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ van Pouderoyen G, Snijder HJ, Benen JA, Dijkstra BW. Structural insights into the processivity of endopolygalacturonase I from Aspergillus niger. FEBS Lett. 2003 Nov 20;554(3):462-6. PMID:14623112
