1f9j
From Proteopedia
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| , resolution 2.70Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF A NEW CRYSTAL FORM OF TETRAUBIQUITIN
Contents |
Overview
Polyubiquitin chains, in which the C-terminus and a lysine side chain of successive ubiquitin molecules are linked by an isopeptide bond, function to target substrate proteins for degradation by the 26S proteasome. Chains of at least four ubiquitin moieties appear to be required for efficient recognition by the 26S proteasome, although the conformations of the polyubiquitin chains recognized by the proteasome or by other enzymes involved in ubiquitin metabolism are currently unknown. A new crystal form of tetraubiquitin, which has two possible chain connectivities that are indistinguishable in the crystal, is reported. In one possible connectivity, the tetraubiquitin chain is extended and packs closely against the antiparallel neighbor chain in the crystal to conceal a hydrophobic surface implicated in 26S proteasome recognition. In the second possibility, the tetraubiqutitin forms a closed compact structure, in which that same hydrophobic surface is buried. Both of these conformations are quite unlike the structure of tetraubiquitin that was previously determined in a different crystal form [Cook et al. (1994), J. Mol. Biol. 236, 601--609]. The new structure suggests that polyubiquitin chains may possess a substantially greater degree of conformational flexibility than has previously been appreciated.
Disease
Known disease associated with this structure: Cleft palate, isolated OMIM:[191339]
About this Structure
1F9J is a Single protein structure of sequence from Homo sapiens. The following page contains interesting information on the relation of 1F9J with [Ubiquitin]. Full crystallographic information is available from OCA.
Reference
Structure of a new crystal form of tetraubiquitin., Phillips CL, Thrower J, Pickart CM, Hill CP, Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):341-4. PMID:11173499
Page seeded by OCA on Thu Mar 20 11:06:14 2008
