1mw9 is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In order to gain insights into the mechaism of ssDNA binding and recognition by Escherichia coli DNA topoisomerase I, the structure of the 67 kDa N-terminal fragment of topoisomerase I was solved in complex with ssDNA. The structure reveals a new conformational stage in the multistep catalytic cycle of type IA topoisomerases. In the structure, the ssDNA binding groove leading to the active site is occupied, but the active site is not fully formed. Large conformational changes are not seen; instead, a single helix parallel to the ssDNA binding groove shifts to clamp the ssDNA. The structure helps clarify the temporal sequence of conformational events, starting from an initial empty enzyme and proceeding to a ssDNA-occupied and catalytically competent active site.
Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA.,Perry K, Mondragon A Structure. 2003 Nov;11(11):1349-58. PMID:14604525[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Perry K, Mondragon A. Structure of a complex between E. coli DNA topoisomerase I and single-stranded DNA. Structure. 2003 Nov;11(11):1349-58. PMID:14604525
