Assembly of the human signal recognition particle (SRP) requires SRP19 protein to bind to helices 6 and 8 of SRP RNA. In the present study, structure of a 29-mer RNA composing the SRP19 binding site in helix 6 was determined by NMR spectroscopy. The two A:C mismatches were continuously stacked to each other and formed wobble type A:C base pairs. The GGAG tetraloop in helix 6 was found to adopt a similar conformation to that of GNRA tetraloop, suggesting that these tetraloops are included in an extensive new motif GNRR. Compared with the crystal structure of helix 6 in complex with SRP19 determined previously, the GGAG tetraloop in the complex was found to adopt a similar conformation to the free form, although the loop structure becomes more open upon SRP19 binding. Thus, SRP19 is thought to recognize the overall fold of the GGAG loop.
Solution structure of a SRP19 binding domain in human SRP RNA.,Sakamoto T, Morita S, Tabata K, Nakamura K, Kawai G J Biochem. 2002 Aug;132(2):177-82. PMID:12153712[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Sakamoto T, Morita S, Tabata K, Nakamura K, Kawai G. Solution structure of a SRP19 binding domain in human SRP RNA. J Biochem. 2002 Aug;132(2):177-82. PMID:12153712
