1uky is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Two crystal structures of ligated uridylate kinase from Saccharomyces cerevisiae were determined by X-ray analyses. The ligands were ADP and AMP. Cocrystallization with ATP yielded crystals with ADP at the ATP site and a mixture of AMP and ADP at the NMP site. Cocrystallization with ADP gave rise to a distinct crystal type with ADP at the ATP site, but only AMP at the NMP site. In both cases, the substrates are kept in place by favorable crystal contacts. The structures have been refined to R-factors of 17.8% and 19.6% at resolutions of 2.1 A and 1.9 A, respectively. A comparison with the related cytosolic adenylate kinase from pig disclosed large induced-fit movements on substrate binding and the disassembly of the catalytic center in the absence of substrates. The relatively high side-activity of uridylate kinase for AMP is explained by the finding that the binding pocket is sized for an AMP, but constructed to bind UMP together with a water molecule.
Substrate specificity and assembly of the catalytic center derived from two structures of ligated uridylate kinase.,Muller-Dieckmann HJ, Schulz GE J Mol Biol. 1995 Mar 3;246(4):522-30. PMID:7877173[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Muller-Dieckmann HJ, Schulz GE. Substrate specificity and assembly of the catalytic center derived from two structures of ligated uridylate kinase. J Mol Biol. 1995 Mar 3;246(4):522-30. PMID:7877173
