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1rif
From Proteopedia
Revision as of 20:14, 28 September 2014 by OCA (Talk | contribs)
1rif is a 2 chain structure with sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
In bacteriophage T4, the WXY system repairs DNA damage by a process that involves homologous recombination. This system comprises three proteins, the RecA-like recombination protein UvsX, a recombination mediator protein UvsY, and a helicase UvsW. Here we report the 2.0 A resolution crystal structure of the N-terminal two domains of the UvsW helicase (UvsWNF; residues 1-282). The structure reveals a typical helicase RecA-like domain linked to a small N-terminal alpha/beta domain that likely binds the nucleic acid substrate. The missing C-terminal portion of UvsW almost certainly corresponds to the second RecA-like domain typically found in monomeric helicases. The putative substrate binding domain is unique within the known helicase structures, and it resembles the novel "double-wing" DNA binding domain from the phage T4 MotA transcription factor that mediates the expression of T4 middle genes. The functional implications of this homology for the role of UvsW in T4 DNA metabolism are discussed.
The crystal structure of the UvsW helicase from bacteriophage T4.,Sickmier EA, Kreuzer KN, White SW Structure. 2004 Apr;12(4):583-92. PMID:15062081[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Sickmier EA, Kreuzer KN, White SW. The crystal structure of the UvsW helicase from bacteriophage T4. Structure. 2004 Apr;12(4):583-92. PMID:15062081 doi:10.1016/j.str.2004.02.016
