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1rnh
From Proteopedia
Revision as of 21:38, 28 September 2014 by OCA (Talk | contribs)
1rnh is a 1 chain structure with sequence from Escherichia coli k-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Ribonuclease H digests the RNA strand of duplex RNA.DNA hybrids into oligonucleotides. This activity is indispensable for retroviral infection and is involved in bacterial replication. The ribonuclease H from Escherichia coli is homologous with the retroviral proteins. The crystal structure of the E. coli enzyme reveals a distinctive alpha-beta tertiary fold. Analysis of the molecular model implicates a carboxyl triad in the catalytic mechanism and suggests a likely mode for the binding of RNA.DNA substrates. The structure was determined by the method of multiwavelength anomalous diffraction (MAD) with the use of synchrotron data from a crystal of the recombinant selenomethionyl protein.
Structure of ribonuclease H phased at 2 A resolution by MAD analysis of the selenomethionyl protein.,Yang W, Hendrickson WA, Crouch RJ, Satow Y Science. 1990 Sep 21;249(4975):1398-405. PMID:2169648[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Yang W, Hendrickson WA, Crouch RJ, Satow Y. Structure of ribonuclease H phased at 2 A resolution by MAD analysis of the selenomethionyl protein. Science. 1990 Sep 21;249(4975):1398-405. PMID:2169648
