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1t8k
From Proteopedia
Revision as of 22:55, 28 September 2014 by OCA (Talk | contribs)
1t8k is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A topic of current interest is engineering surface mutations in order to improve the success rate of protein crystallization. This report explores the possibility of using metal-ion-mediated crystal-packing interactions to facilitate rational design. Escherichia coli apo acyl carrier protein was chosen as a test case because of its high content of negatively charged carboxylates suitable for metal binding with moderate affinity. The protein was successfully crystallized in the presence of zinc ions. The crystal structure was determined to 1.1 A resolution with MAD phasing using anomalous signals from the co-crystallized Zn(2+) ions. The case study suggested an integrated strategy for crystallization and structure solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and solving the structures using anomalous signals.
Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions.,Qiu X, Janson CA Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:15333924[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Qiu X, Janson CA. Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions. Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:15333924 doi:10.1107/S0907444904015422
