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1pd3
From Proteopedia
Revision as of 00:02, 29 September 2014 by OCA (Talk | contribs)
1pd3 is a 2 chain structure with sequence from Influenza a virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
During influenza virus infection, viral ribonucleoproteins (vRNPs) are replicated in the nucleus and must be exported to the cytoplasm before assembling into mature viral particles. Nuclear export is mediated by the cellular protein Crm1 and putatively by the viral protein NEP/NS2. Proteolytic cleavage of NEP defines an N-terminal domain which mediates RanGTP-dependent binding to Crm1 and a C-terminal domain which binds to the viral matrix protein M1. The 2.6 A crystal structure of the C-terminal domain reveals an amphipathic helical hairpin which dimerizes as a four-helix bundle. The NEP-M1 interaction involves two critical epitopes: an exposed tryptophan (Trp78) surrounded by a cluster of glutamate residues on NEP, and the basic nuclear localization signal (NLS) of M1. Implications for vRNP export are discussed.
Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2).,Akarsu H, Burmeister WP, Petosa C, Petit I, Muller CW, Ruigrok RW, Baudin F EMBO J. 2003 Sep 15;22(18):4646-55. PMID:12970177[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Akarsu H, Burmeister WP, Petosa C, Petit I, Muller CW, Ruigrok RW, Baudin F. Crystal structure of the M1 protein-binding domain of the influenza A virus nuclear export protein (NEP/NS2). EMBO J. 2003 Sep 15;22(18):4646-55. PMID:12970177 doi:http://dx.doi.org/10.1093/emboj/cdg449
