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1orc
From Proteopedia
Revision as of 00:14, 29 September 2014 by OCA (Talk | contribs)
1orc is a 1 chain structure with sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A rationally designed, genetically engineered, monomeric form of the Cro protein from bacteriophage lambda has been crystallized and its structure determined by isomorphous replacement and refined to a resolution of 1.54 A. The structure confirms the rationale of the design but, at the same time, reveals 1-2 A shifts throughout the monomer structure relative to the previously determined structure of the dimeric wild-type protein. These changes include a 1.6 A main-chain shift in part of the beta-sheet region of the molecule relative to the alpha-helical region and a 1.1 A shift of a buried phenylalanine within the core as well as a correlated 2.2 A shift in a solvent-exposed beta-hairpin. The conformational adjustments appear to reflect an inherent flexibility of the protein that is associated with its DNA-binding function.
High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer.,Albright RA, Mossing MC, Matthews BW Biochemistry. 1996 Jan 23;35(3):735-42. PMID:8547253[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Albright RA, Mossing MC, Matthews BW. High-resolution structure of an engineered Cro monomer shows changes in conformation relative to the native dimer. Biochemistry. 1996 Jan 23;35(3):735-42. PMID:8547253 doi:http://dx.doi.org/10.1021/bi951958n
