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2hnk
From Proteopedia
Revision as of 04:39, 29 September 2014 by OCA (Talk | contribs)
2hnk is a 3 chain structure with sequence from Leptospira interrogans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The S-adenosylmethionine (SAM)-dependent O-methyltransferase from Leptospira interrogans (LiOMT) expressed by gene LA0415 belongs to the Methyltransf_3 family (Pfam PF01596). In this family all of the five bacterial homologues with known function are reported as SAM-dependent O-methylstransferases involved in antibiotic production. The crystal structure of LiOMT in complex with S-adenosylhomocysteine reported here is the first bacterial protein structure in this family. The LiOMT structure shows a conserved SAM-binding region and a probable metal-dependent catalytic site. The molecules of LiOMT generate homodimers by N-terminal swapping, which assists the pre-organization of the substrate-binding site. Based on the sequence and structural analysis, it is implied by the catalytic and substrate-binding site that the substrate of LiOMT is a phenolic derivative, which probably has a large ring-shaped moiety.
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans.,Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:17561415[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W. Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans. J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:17561415 doi:10.1016/j.jsb.2007.04.007
