We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.
2i2u
From Proteopedia
Revision as of 05:05, 29 September 2014 by OCA (Talk | contribs)
Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 30s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.
2i2u is a 23 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.
Structural basis for mRNA and tRNA positioning on the ribosome.,Berk V, Zhang W, Pai RD, Cate JH Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:17038497[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
↑ Berk V, Zhang W, Pai RD, Cate JH. Structural basis for mRNA and tRNA positioning on the ribosome. Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:17038497
