| Structural highlights
2wrn is a 26 chain structure with sequence from Escherichia coli and Thermus thermophilus. The January 2010 RCSB PDB Molecule of the Month feature on 70S Ribosomes by David Goodsell is 10.2210/rcsb_pdb/mom_2010_1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , ,
| | NonStd Res: | , , , , |
| Related: | 2wro, 2wrq, 2wrr, 1ibk, 1ibl, 2uuc, 2wdk, 2j02, 1j5e, 2j00, 1hnx, 2b9o, 2wh3, 2uub, 1vov, 1emi, 1pnx, 2v46, 2vqf, 2wh1, 1n32, 2f4v, 2jl5, 1twt, 1qzc, 2uxd, 1fka, 1fjg, 1n36, 2uxc, 1hnw, 1l1u, 1hr0, 2v48, 2b64, 1xmq, 2b9m, 1pn8, 2wdg, 1xnr, 1hnz, 1dv4, 1rss, 2wdh, 1jgq, 1i94, 1i96, 2uu9, 1pn7, 1eg0, 1ibm, 2uxb, 2vqe, 1g1x, 1ha3, 2uua, 1i95, 1xnq, 1qd7, 1gix, 1jgo, 1jgp, 2c78, 1n34, 1n33, 1pns, 2jl7, 1yl4, 2wdm, 1xmo, 1i97, 2wrj, 2wri, 2wrk, 2wrl |
| Activity: | Protein-synthesizing GTPase, with EC number 3.6.5.3 |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The ribosome selects a correct transfer RNA (tRNA) for each amino acid added to the polypeptide chain, as directed by messenger RNA (mRNA). Aminoacyl-tRNA is delivered to the ribosome by elongation factor Tu (EF-Tu), which hydrolyzes guanosine triphosphate (GTP) and releases tRNA in response to codon recognition. The signaling pathway that leads to GTP hydrolysis upon codon recognition is critical to accurate decoding. Here, we present the crystal structure of the ribosome complexed with EF-Tu and aminoacyl-tRNA, refined to 3.6 angstrom resolution. The structure reveals details of the tRNA distortion that allows aminoacyl-tRNA to interact simultaneously with the decoding center of the 30S subunit and EF-Tu at the factor-binding site. A series of conformational changes in EF-Tu and aminoacyl-tRNA suggest a communication pathway between the decoding center and the guanosine triphosphatase center of EF-Tu.
The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA.,Schmeing TM, Voorhees RM, Kelley AC, Gao YG, Murphy FV 4th, Weir JR, Ramakrishnan V Science. 2009 Oct 15. PMID:19833920[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Schmeing TM, Voorhees RM, Kelley AC, Gao YG, Murphy FV 4th, Weir JR, Ramakrishnan V. The Crystal Structure of the Ribosome Bound to EF-Tu and Aminoacyl-tRNA. Science. 2009 Oct 15. PMID:19833920
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