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3i17

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Crystal structure of the apo R132K:L121E mutant of cellular retinoic acid-binding protein II at 1.68 anstrom resolution

Structural highlights

3i17 is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2fs6, 2fs7, 3fa7
Gene:	CRABP2 (Homo sapiens)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Cellular Retinoic Acid Binding Protein II (CRABPII) has been reengineered to specifically bind and react with all-trans-retinal to form a protonated Schiff base. Each step of this process has been dissected and four residues (Lys132, Tyr134, Arg111, and Glu121) within the CRABPII binding site have been identified as crucial for imine formation and/or protonation. The precise role of each residue has been examined through site directed mutagenesis and crystallographic studies. The crystal structure of the R132K:L121E-CRABPII (PDB-3I17) double mutant suggests a direct interaction between engineered Glu121 and the native Arg111, which is critical for both Schiff base formation and protonation. Proteins 2009. (c) 2009 Wiley-Liss, Inc.

Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base.,Vasileiou C, Wang W, Jia X, Lee KS, Watson CT, Geiger JH, Borhan B Proteins. 2009 Jun 8. PMID:19603486^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vasileiou C, Wang W, Jia X, Lee KS, Watson CT, Geiger JH, Borhan B. Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base. Proteins. 2009 Jun 8. PMID:19603486 doi:10.1002/prot.22495

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

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3i17

From Proteopedia

Crystal structure of the apo R132K:L121E mutant of cellular retinoic acid-binding protein II at 1.68 anstrom resolution

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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