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1qj4
From Proteopedia
Revision as of 15:04, 29 September 2014 by OCA (Talk | contribs)
1qj4 is a 1 chain structure with sequence from Hevea brasiliensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The X-ray crystal structure of native hydroxynitrile lyase from Hevea brasiliensis (Hb-HNL) has been determined at 1.1 A resolution. It refined to a final R of 11.5% for all data and an Rfree of 14.4%. The favorable data-to-parameter ratio at atomic resolution made the refinement of individual anisotropic displacement parameters possible. The data also allowed a clear distinction of the alternate orientations of all histidine and the majority of asparagine and glutamine side chains. A number of hydrogen atoms, including one on the imidazole of the mechanistically important His-235, became visible as peaks in a difference electron density map. The structure revealed a discretely disordered sidechain of Ser-80, which is part of the putative catalytic triad. Analysis of the anisotropy indicated an increased mobility of residues near the entrance to the active site and within the active site.
Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis.,Gruber K, Gugganig M, Wagner UG, Kratky C Biol Chem. 1999 Jul-Aug;380(7-8):993-1000. PMID:10494852[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Gruber K, Gugganig M, Wagner UG, Kratky C. Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis. Biol Chem. 1999 Jul-Aug;380(7-8):993-1000. PMID:10494852
