| Structural highlights
1gxw is a 1 chain structure with sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , , , ,
| | Related: | 1fj3, 1fjo, 1fjq, 1fjt, 1fju, 1fjv, 1fjw, 1hyt, 1lna, 1lnb, 1lnc, 1lnd, 1lne, 1lnf, 1qf0, 1qf1, 1qf2, 1thl, 1tli, 1tlp, 1tlx, 1tmn, 1trl, 2tli, 2tlx, 2tmn, 3tli, 3tmn, 4tli, 4tln, 4tmn, 5tli, 5tln, 5tmn, 6tli, 6tmn, 7tli, 7tln, 8tli, 8tln |
| Activity: | Thermolysin, with EC number 3.4.24.27 |
| Resources: | FirstGlance, OCA, RCSB, PDBsum |
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
A new crystallization protocol for thermolysin (EC 3.4.24.27) from Bacillus thermoproteolyticus is presented. After dissolving the protein in the presence of KSCN, which avoids the use of DMSO and CsCl, crystals were obtained following the salting-in method. Crystal cell parameters are isomorphous with those previously reported from DMSO/CsCl mixtures. The new SCN(-) crystal structure has been analyzed. It shows the presence of one thiocyanate ion in the catalytic site and several rearrangements in the S(1) and S(2) subsites. These results are in agreement with the measurements of Inouye et al. [(1998), J. Biochem. (Tokyo), 123, 847-852], who observed in solution that the solubility of TLN, which is particularly poor in low ionic strength solutions, increases dramatically in the presence of several neutral salts. The results reported here suggest possible explanations for the solubility increase and for the inhibitory effects of high SCN(-) concentrations on thermolysin activity.
The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate.,Gaucher JF, Selkti M, Prange T, Tomas A Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:12454500[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Gaucher JF, Selkti M, Prange T, Tomas A. The 2.2 A resolution structure of thermolysin (TLN) crystallized in the presence of potassium thiocyanate. Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2198-200. Epub, 2002 Nov 23. PMID:12454500
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