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1z9o
From Proteopedia
Revision as of 19:09, 29 September 2014 by OCA (Talk | contribs)
1z9o is a 12 chain structure with sequence from Rattus norvegicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The FFAT motif is a targeting signal responsible for localizing a number of proteins to the cytosolic surface of the endoplasmic reticulum (ER) and to the nuclear membrane. FFAT motifs bind to members of the highly conserved VAP protein family, which are tethered to the cytoplasmic face of the ER by a C-terminal transmembrane domain. We have solved crystal structures of the rat VAP-A MSP homology domain alone and in complex with an FFAT motif. The co-crystal structure was used to design a VAP mutant that disrupts rat and yeast VAP-FFAT interactions in vitro. The FFAT binding-defective mutant also blocked function of the VAP homolog Scs2p in yeast. Finally, overexpression of the FFAT binding-defective VAP in COS7 cells dramatically altered ER morphology. Our data establish the structural basis of FFAT-mediated ER targeting and suggest that FFAT-targeted proteins play an important role in determining ER morphology.
Structural basis of FFAT motif-mediated ER targeting.,Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT Structure. 2005 Jul;13(7):1035-45. PMID:16004875[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
↑ Kaiser SE, Brickner JH, Reilein AR, Fenn TD, Walter P, Brunger AT. Structural basis of FFAT motif-mediated ER targeting. Structure. 2005 Jul;13(7):1035-45. PMID:16004875 doi:10.1016/j.str.2005.04.010
