Publication Abstract from PubMed
We describe here the crystal structure of the trichosanthin-NADPH complex determined at a resolution of 1.7 A. The adenine base stacks between Tyr 70 and Tyr 111. Arg 163, Glu 160 and Tyr 70 form hydrogen bonds to N(3), O(3') and, through a water molecule, to N(9) of adenosine, respectively. This is the first high resolution structure of a complex between a ribosome-inactivating protein and a substrate analogue, in which the electron density of the N-glycosidic bond is well defined and the preassociated water, thought to be responsible for hydrolyzing the N-C bond, is also explicitly elucidated.
Crystal structure of trichosanthin-NADPH complex at 1.7 A resolution reveals active-site architecture.,Xiong JP, Xia ZX, Wang Y Nat Struct Biol. 1994 Oct;1(10):695-700. PMID:7634073[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
